Chunk #6 — 2. Materials and Methods — 2.3 Experimental procedure — 2.3.1 DNA extraction and bisulfite modification

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CHN2 Promoter Methylation Change May Be Associated With Methamphetamine Dependence.

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10 ml of peripheral blood was extracted and DNA was extracted. The DNA was modified with the EZ DNA Methylation-GoldTM Kit to convert the unmethylated cytosine (C) in the DNA sequence to uracil (U).