Vanlandewijck et al. described two brain fibroblast-like cell types expressing fibril-forming collagens (e.g., Col1a1, Col1a2), collagen fiber crosslinking proteins (Lum, Dcn), and the platelet-derived growth factor receptor alpha, Pdgfra, which was interposed between astrocyte endfeet and vascular endothelial cells. Brain fibroblast-like cells are likely identical to the vascular leptomeningeal cells (VLMCs) that we previously described in the mouse CNS (Marques et al., 2016). In the present dataset, we observed four types sharing the canonical markers. Two types were distinguished by expression of genes including the pro-inflammatory cytokine Il33 (VLMC1) and the Prostaglandin D2 synthetase Ptgds (VLMC2), the latter previously found as the most highly enriched gene in mouse leptomeninges (Yasuda et al., 2013) (Figure S3A).
