volume of fluid consumed, alcohol dose (g/kg/day) and alcohol and saccharin preference. Two-bottle choice drinking ended 24h prior to the first behavioral test day (open field activity). During all behavioral testing, which occurred during 1–3 weeks post alcohol, mice had ad libitum access to water only (Fig. 3). Behavioral testing occurred in the following sequence with a minimum of 1 day in between each test: open field locomotion, rotarod, Morris Water Maze, prepulse inhibition, fear conditioning.
