Mice were singly housed for 2 weeks prior to initiation of home-cage alcohol drinking studies. Voluntary alcohol drinking studies were conducted as previously reported in Hodge et al. (1999); Ueno et al. (2001); Besheer, Lepoutre, Mole, and Hodge (2006); Stevenson et al. (2009); Holstein, Spanos, and Hodge (2011); Agoglia, Holstein, et al. (2015); Agoglia, Holstein, Eastman, and Hodge (2016); Salling et al. (2016); and Faccidomo et al. (2018). Briefly, mice were given continuous 24h access to two 50mL bottles containing sweetened alcohol (25%, w/v + saccharin 0.1%, w/v) or water for 4 months (120 days). Parallel control groups had continuous access to 2 bottle containing saccharin (0.1% w/v) or water. Fluid consumed was measured every 48h and the position of the bottles (left or right) was alternated to avoid a side preference. Food was available ad libitum. Dependent measures included volume of fluid consumed, alcohol dose (g/kg/day) and alcohol and saccharin preference. Two-bottle choice drinking ended 24h prior to the first behavioral test day (open field activity). During all behavioral testing, which occurred during 1–3 weeks post alcohol, mice had
