Varying amounts of pulverized cerebellar tissue were used (45 to 80 mg) for DNA extraction. The QIAamp DNA mini Kit (Qiagen) method was used. The tissue was initially lysed using Tissue Lyser (Qiagen) and extractions proceeded according to manufacturer’s protocol. DNA was captured in 500uL elution buffer. The concentrations were measured using Thermo Scientific’s NanoDrop 1000/NanoDrop ONE. The mean yield was 128.85 uG (+/−79.48), the mean ratio of 260/280 was 1.87 (+/−0.105), and the mean ratio of 260/230 was 2.48 (+/−1.75).
