DNA was isolated from approximately 10 mg dry homogenized tissue coming from the same dissected samples as the RNA isolation. The thinly sliced tissue from Pitt was homogenized before DNA isolation. All DNA isolation was done using the Qiagen DNeasy Blood and Tissue Kit according to manufacturer’s protocol. DNA yield was quantified using Thermo Scientific’s NanoDrop. The mean yield was 12.6 ug (+/−4.6), the mean ratio of 260/280 was 2.0 (+/−0.1) and the mean ratio of 260/230 was 1.9 (+/−2.3).
