PVBCs express highest level of Cplx1 but lowest level of Cplx2, while VIP/CCK celld show the opposite (Figure 6H–J). This Cplx profile is highly congruent with the Syt profile, as Cplx1 is implicated in fast and synchronous release and in clamping spontaneous release (Yang et al., 2013). These results suggest that fast-synchronous release is supported by high levels of VAMP1, SNAP25, NSF, SYT2, CPLX1, whereas slow-asynchronous release is shaped with low levels of these components and high levels of CPLX2. Together with co-expression of matching properties of Ca2+ channels and CaBPs, these results suggest that PCPs might transmit multiple neurochemicals in multiple release styles through differential and coordinated expression of gene families that customize the vesicle fusion machinery (Table S7).
