The release sites (e.g. synaptic vs non-synaptic), temporal characteristics (e.g. fast-synchronous vs slow-asynchronous) and short term dynamics (facilitating vs depressing) of GABA and neuropeptides produce distinct spatiotemporal patterns of receptor activation and postsynaptic cell firing that impact circuit computation (Armstrong and Soltesz, 2012; Markram et al., 2015). Our analyses begin to reveal molecular distinctions of fast-synchronous (in PVBCs) vs slow-sustained release (in CCKBCs) machinery, which manifests even at the level of core fusion complex. Vamp1, Snap25, Nsf and Rab3a are highly enriched in PV compared to VIP/CCK cells (Figure 6G–H); co-expression of all three components in PVBCs supports their fast release properties (Parpura and Mohideen, 2008). Among the Ca2+-binding Syts localized to SVs (Syt1, 2, 9), both PV and VIP/CCK cells (and other PCPs) express Syt1, but only PVBCs express Syt2, which exhibits the fastest onset and decline in release. PVBCs express highest level of Cplx1 but lowest level of Cplx2, while VIP/CCK celld show the opposite (Figure 6H–J). This Cplx profile is highly congruent with the Syt profile, as Cplx1 is implicated in fast and synchronous release and in
