The ALDH1A1 promoter region polymorphism (rs8187866; alleles ALDH1A*1, ALDH1A*2 (17 base-pair deletion) and ALDH1A1*3 (3 base-pair insertion)) was genotyped on the ABI 310 Genetic Analyzer using the A4-forward and A4-reverse primers of Spence and colleagues (2003), with a 5′-FAM-fluorescent dye added to the A4-forward primer. The size estimates for the alleles using the Applied Biosystems Genescan-500 size standard were about 6 basepairs larger (198, 215, 218 bp vs. 192, 209, 212 bp) than Spence and colleagues (2003).
