Likewise, the ADH1C haplotypes were determined by separate PCR amplifications of the regions around exon 6 (Arg272Gln; rs1693482; PCR primers from sequencing protocol of ss8819648) and exon 8 (Ile350Val; rs698; primers from Osier et al, 2002), followed by restriction endonuclease digests (with Alu I and Ssp I, respectively). These sites are known to be in strong linkage disequilibrium, and, indeed, all samples were either homozygous for both Arg (exon 6) and Ile (exon 8), homozygous for both Gln (exon 6) and Val (exon 8), or were heterozygous at both sites. Thus, haplotypes ADH1C*1 (272Arg-350Ile) and ADH1C*2 (272Gln-350Val) were determined.
