Buccal brush samples were sent to Washington University School of Medicine for genotype analysis. The ADH1B haplotypes were determined by separate PCR amplifications (primers from Osier et al, 2002) of the regions around exon 3 (Arg48His; rs1229984) and exon 9 (Arg370Cys; rs2066702) followed by restriction endonuclease digests (with Msl I and Alw NI, respectively). No samples were heterozygous at both sites, so the haplotypes ADH1B*1 (48Arg-370Arg), ADH1B*2 (48His-370Arg), and ADH1B*3 (48Arg-370Cys) were clearly indicated.
