Chunk #29 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.4. Puromycin withdrawal and switch to neuron medium — Day 6–20: Ara-C treatment

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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Neuron medium can be supplemented with 50 nM Cytosineβ-D-arabinofuranoside hydrochloride (Ara-C) (Sigma, #C6645) to reduce the proliferation of non-neuronal cells in the culture (Fig. 4F–H).