Chunk #28 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.4. Puromycin withdrawal and switch to neuron medium — Day 4–20: Regular half medium changes
To achieve this, 250 μl of medium per well was removed and replaced with 280 μl of fresh neuron medium (the added volume is greater than the removed volume in order to prevent gradual loss of the media volume by evaporation). Cells were fed every second day by half medium change until the day of assay.
