Chunk #27 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.4. Puromycin withdrawal and switch to neuron medium — Day 4–20: Regular half medium changes
Doxycycline can be withdrawn at any time during the protocol. We recommend a 50% reduction in the total doxycycline concentration every 48 h, which will result in a complete removal from the culture medium by day 7–8 (Fig. 5A).
