Chunk #26 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.4. Puromycin withdrawal and switch to neuron medium — Day 2: Puromycin withdrawal and switch to neuron medium

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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Media was replaced with neuron medium (Table 1) containing 1 μg/ml doxycycline (500 μl per well of a 24-well plate).