Chunk #25 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.3. Doxycycline addition and puromycin selection — Day 1: Puromycin selection

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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Media was replaced with fresh NPC medium containing 1 μg/ml puromycin (Sigma, #P7255) and 1 μg/ml doxycycline for 24 h.