Chunk #24 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.3. Doxycycline addition and puromycin selection — Day 0: Doxycycline treatment

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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24 h following doxycycline addition, GFP fluorescence can be observed in most NPCs (usually >90%, Fig. 1D).