For immunofluorescence experiments, cells were fixed in 4% paraformaldehyde in PBS for 10 min at room temperature. Following fixation, cells were incubated in 0.2% Triton X-100 in PBS for 5 min at room temperature. After washing twice with PBS, cells were blocked in a solution of PBS containing 4% BSA and 1% cosmic calf serum (CCS) for 30 minutes at room temperature. Primary and secondary antibodies were applied for 1 hour and 30 min, respectively. The following antibodies were used for our analysis: rabbit anti-Tuj1 (Covance, 1:1000), mouse anti-Tuj1 (Covance, 1:1000), mouse anti-MAP2 (Sigma, 1:500), mouse anti-NeuN (Millipore, 1:200), mouse anti-neurofilament (Developmental Studies Hybridoma Bank (DSHB), 2H3a, 1:1000), rabbit anti-synapsin (E028, 1:1000), anti-synaptotagmin (Synaptic systems, 41.1, 1:2000), guinea pig anti-vGLUT1 (Millipore, 1:2000), mouse anti-GAD6 (DSHB, 1:500), rabbit anti-Tbr1 (Abcam, 1:200), mouse anti-Peripherin (Sigma, 1:100), sheep anti-Tyrosine Hydroxylase (Pel-Freez, 1:500), rabbit anti-GFAP (DAKO, 1:4000), mouse anti-Sox2 (R&D Systems, 1:50), goat anti-Brn2 (clone C-20, Santa Cruz Biotechnology, 1:100), rabbit anti-Ascl1 (Abcam, 1:200), mouse anti-BrdU (Becton-Dickinson, 1:50), mouse anti-LU5 (Abcam, 1:200), goat anti-Sox10 (Santa Cruz Biotechnology, 1:40). FITC-, and Cy3-conjugated secondary antibodies were
