anti-GFAP (DAKO, 1:4000), mouse anti-Sox2 (R&D Systems, 1:50), goat anti-Brn2 (clone C-20, Santa Cruz Biotechnology, 1:100), rabbit anti-Ascl1 (Abcam, 1:200), mouse anti-BrdU (Becton-Dickinson, 1:50), mouse anti-LU5 (Abcam, 1:200), goat anti-Sox10 (Santa Cruz Biotechnology, 1:40). FITC-, and Cy3-conjugated secondary antibodies were obtained from Jackson Immunoresearch. Alexa-488-, Alexa-546- and Alexa-633-conjugated secondary antibodies were obtained from Invitrogen. DAPI (Sigma, 1:10,000). For RT-PCR analysis, RNA was isolated using RNAqueous® Kit (Applied Biosystems) following the manufacturer’s instructions, treated with DNAse (Applied Biosystems) and reverse-transcribed with Superscript III (Invitrogen).
