Chunk #52 — Materials and methods — Mutation detection procedure — Step 2a: filtering of candidate mutations — Filter 2

Source

Detection of low prevalence somatic mutations in solid tumors with ultra-deep targeted sequencing.

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yes

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Any position with a fraction of non-reference alleles less than 0.2% of the total coverage at this position is filtered out. This threshold was used based on the initial specification of UDT-Seq to identify variants present at 1% prevalence. An alternative allele with five times lower support than 1% prevalence is unlikely to be a true positive.