Chunk #53 — Materials and methods — Mutation detection procedure — Step 2a: filtering of candidate mutations — Filter 3

Source

Detection of low prevalence somatic mutations in solid tumors with ultra-deep targeted sequencing.

Embedded

yes

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For positions covered by a single read (forward or reverse), we reject all positions located more than 100 nucleotides from the read start, to prevent increasing error rate at the end of the reads.