Chunk #32 — 2. Material and methods — 2.3. Analysis of mNgn2- and hNGN2-induced neurons — 2.3.1. Immunostaining of mNgn2- and hNGN2-neurons

Source

Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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During blocking and permeabilization, the primary antibody (Table 2) solution was prepared in ice-cold blocking/permeabilization buffer. Neurons were incubated with the primary antibody solution overnight at 4 °C.