Chunk #33 — 2. Material and methods — 2.3. Analysis of mNgn2- and hNGN2-induced neurons — 2.3.1. Immunostaining of mNgn2- and hNGN2-neurons
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Following incubation, the primary antibody solution was removed and the wells were washed with ice-cold PBSCa2+Mg2+ three times as described above.