The effect of AM3506 on glutamatergic transmission in the BLA was tested by measuring fEPSP. Application of AM3506 produced a small, but significant, decrease in fEPSP amplitude (last 5-min time block vs baseline: t(5) = 3.06, P<0.05, n = 6) (Supplementary Figure S5a,b), consistent with a modest decrease in excitatory transmission. Next, we tested the ability of AM3506 pretreatment to gate the induction of excitatory LTD induced by low LFS2 (10 Hz for 10 min) using a protocol described previously.35,36 There was no change in fEPSPs, regardless of drug treatment (Supplementary Figure S5c,d), indicating this form of LTD was unaffected by AM3506 (n = 5–7) (Supplementary Figure S5c,d).
