Fibrillar fluorescent amyloid-beta (fAβ1-42) was generated as described previously (Koenigsknecht-Talboo and Landreth, 2005). Fluorescently labeled Aβ peptide (Anaspec; Fremont, CA) was first dissolved in NH4OH (0.1%) to 1 mg/ml, then further diluted to 100 μg/ml using sterile endotoxin-free water, vortexed thoroughly, and incubated at 37°C for 7 days. fAβ was thoroughly mixed prior to cell exposure.
