iMGLs and MD-Mϕ, were incubated with mouse anti CD16/32 Fc-receptor block (2 mg/ml; BD Biosciences) for 15 minutes at 4°C. Cells were then stained with anti CD45-APC clone HI30 (Tonbo Biosciences) at 1:200 in flow cytometer buffer. Samples were then analyzed using Amnis Imagestreamer®x Mark II Imaging Flow Cytometer (Millipore). E.coli, human synaptosome, fAβ, and BDTO phagocytosis was analyzed using the IDEAS software onboard Internalization Wizard algorithm. Additive free Anti-CD11b antibody (BioLegend, #301312) was used for CD11b blockade.
