Given that repeated, but not acute, restraint stress confers an enhanced capacity to elevate 2-AG levels, we determined the effect of acute and repeated restraint stress on DSI in the BLA. Two-way ANOVA showed a significant effect of stress exposure (p < 0.0001), and time (p < 0.0001), but no significant interaction (p > 0.05). Neither acute nor repeated stress affected maximal DSI, but repeated restraint stress prolonged DSI duration (Figure 5a). Summary data indicate that repeated stress did not affect maximal DSI (control 83.5 ± 4.4% N=17 vs stress 73.9 ± 4.8 N=18, p > 0.05; Figure 5b), but significantly enhanced late DSI (control 99.9 ± 4.0% N=17 vs repeated stress 80.2 ± 3.8% N=18, p < 0.01; Figure 5b). In contrast, a single 60 min restraint stress exposure did not significantly affect either maximal DSI (control 83.5 ± 4.4% N = 17 vs acute stress 86.3 ± 4.5% N = 13, p > 0.05; Figure 5b) or late DSI (control 99.9 ± 4.0% N = 17 vs acute stress 100.6 ± 4.6 N = 13, p < 0.05;
