The effects of systemic AM3506 treatment on anandamide-induced glucose intolerance were tested based on previous methods.19 Because we have shown that another FAAH inhibitor, URB597, produces glucose intolerance in obese mice,19 we examined the effects of URB597, for comparison with AM3506. The mouse was food deprived for 12–16 h and then administered saline vehicle, 1.0 mg/kg AM3506 or 1.0 mg/kg URB597. After 60 min, the mouse received 10 mg/kg anandamide and then, 10 min later, by 1.5 g/kg glucose. Blood glucose levels were measured from blood samples collected from the tail vein, 0,15,30,45, 60, 90 120, 150 and 180 min after glucose administration. The effect of drug treatment was statistically analyzed using a drug × time (repeated measures for time) ANOVA and, for data summarized as an area under the curve, Student's t-test.
