The effects of systemic AM3506 treatment on fasting-induced feeding was tested using methods described previously.32 The mouse was food deprived for 16 h and presented with regular chow in the home cage. The amount of food consumed was measured over 1 h. AM3506 was administered at a dose of 0 or 1.0 mg/kg 60 min before testing. The effect of drug treatment was statistically analyzed using Student's t-test.
