The effects of systemic AM3506 treatment were tested in the forced swim test, a simple screen for antidepressant-related activity.30 The mouse was gently lowered into a 20-cm-diameter cylinder filled with 24±1.0°C water for a 6 min test, as described previously.31 Immobility (cessation of limb movements except minor movement necessary to keep the mouse afloat) was manually scored every 5 s during 3–6 min. AM3506 was administered at a dose of 0 or 1.0 mg/kg 60 min before testing. The effect of drug treatment was statistically analyzed using Student's t-test.
