The effects of systemic AM3506 treatment on locomotor activity were tested in an open field test. The open field was in a 40 × 40 × 35 cm3 square arena (60 lx) constructed of white Plexiglas, as described previously.29 The mouse was placed in the perimeter and allowed to explore the apparatus for 30 min. Total distance traveled and time spent in the center square (20 × 20 cm2) was measured by the Ethovision videotracking system (Noldus Information Technology, Leesburg, VA, USA). AM3506 was administered at a dose of 0 or 1.0 mg/kg 60 min before testing. The effect of drug treatment was statistically analyzed using Student's t-test.
