run in duplicate. To quantify the relative expression levels of the genes for each mouse genotype, we calculated the difference (ΔCt) between the cycle threshold of each gene and the housekeeping gene, GAPDH. From these data, the ΔΔCt ([ΔCtGene(HAP)–ΔCtGene(LAP)]) was computed and converted to a relative quantitative (RQ) value using the formula 2−ΔΔCt. An ANOVA with line (HAP/LAP) and sex (M/F) as factors was used to compare RQ of C5ar2, Pla2g3, Kcnj15, and Ubc using GraphPad Prism (version 8.2). The level of significance was set at p<0.05.
