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Chunk #15 — Materials and Methods — Protein Preparation

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A multi-omic analysis of the dorsal striatum in an animal model of divergent genetic risk for alcohol use disorder.
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Sample preparation, mass spectrometry analysis, bioinformatics, and data evaluation for quantitative proteomics and phosphoproteomics experiments were performed in collaboration with the Proteomics Core Facility at the Indiana University School of Medicine. Methods described below in brief were adaptations from literature reports (Levasseur et al. 2019) and vendor provided protocols.