After 7–21 days of subculture, many of the aDRG and eDRG NSCs expressed the neuroepithelial marker, nestin, in the long, thin extending fibers (Fig. 2C and D for eDRG and aDRG NSCs, respectively). MAP-2abc staining on the soma, axon, and dendrites of neuronal-like cells (for both immature and mature neurons) was positive in differentiated eDRG NSC cultures (Fig. 3A) and was evident in differentiated aDRG NSCs (Fig. 3B). β-III-Tubulin immunostaining [immature and mature neuronal-like cells (28)] was seen in the soma and dendrites of neuronal-like cells derived from eDRG NSCs (Fig. 3C) and aDRG NSCs (Fig. 3D).
