BrdU immunocytochemistry revealed that proliferation occurred during the 48 h of BrdU exposure. The robust BrdU immunostaining present in eDRG NSCs (Fig. 2A) is in contrast to relatively sparse BrdU immunostaining that was present in aDRG NSCs (Fig. 2B). Approximately 10 times as many cells had BrdU immunostaining in eDRG NSC cultures compared to their adult counterparts. Differentiation of early migrating cells began within hours after subplating. After 48 h, both neuronal- and glial-like morphology were seen in both eDRG and aDRG NSCs.
