The clonal analysis from dissociated cells indicted that clonal neurospheres were first evident after 7 days in culture; between days 14 and 21, 50% of the wells contained at least one neurosphere; in some wells, up to four neurospheres were found. After 21 days, 55% of wells contained neurospheres, which averaged 3 ± 1 (mean ± SEM) neurospheres formed per well. These results demonstrate that a small percentage of aDRG NSCs are capable of clonal neurosphere formation.
