DNA was extracted from blood and genotypes were determined by TaqMan® technology. The TaqMan® Pre-Designed SNP Genotyping Assay catalog numbers (Applied Biosystems (ABI), Foster City, CA) are listed in Supplement Table 1. PCR was performed in duplicate on a GeneAmp® PCR 9700 using TaqMan® universal PCR master mix with AmpErase uracil-N-glycosylate (ABI) according to the manufacturer’s protocol. Briefly, TaqMan® assay mix (40x) and universal PCR master mix (2x) were mixed and the volume was adjusted to 4 μl in a 384-well optical reaction plate; 10 ng of genomic DNA (1 μl) were added. PCR amplification lasted for 2 min at 50°C; 10 min at 95°C followed by 40 cycles of 15 sec at 92°C and 1 min at 60°C. Genotype analysis was performed on an ABI Prism® 7900 sequence detection system using SDS 2.1 software (ABI).
