The infusate was prepared by the Indiana University Hospital research pharmacy. Infusion rates were computed and delivered by our Computer-assisted Alcohol infusion System (CAIS, Zimmermann et al., 2008; Plawecki et al. 2012, 2013). An individualized infusion rate profile was pre-calculated using a transformation of the subject’s age, height, weight and gender into the parameters of a physiologically-based pharmacokinetic model of alcohol distribution and elimination (O’Connor et al., 1998; Plawecki et al., 2008; Ramchandani et al., 1999). A computerized algorithm generated an infusion rate profile that, when coupled with automated adjustments based on real-time BrAC measurements, achieved induction and maintenance of a fixed BrAC of 60.0 ± 0.2 mg/dL (Mean ± SEM) from the beginning of Block 1 to the end of Block 2. BrAC measurements were taken with an Alcotest meter, model 7410 or 6510 (Draeger, Irving, Texas).
