For image acquisition during task behavior, mice were briefly anaesthetized using a mixture of isofluorane (0.5%–1%) and oxygen (1 l.min-1) and the miniaturized microscope was attached and secured to the baseplate. This was followed by a 20-30 min period of recovery in the home cage before imaging experiments. Image acquisition (nVistaHD, Inscopix) was done at 10 Hz, with LED power set to 10%–30% (0.1-0.3 mW) with a gain of 3. Image acquisition parameters were set to the same values between sessions for each mouse.
