Three to four weeks after surgery, mice were anaesthetized and placed in the stereotactic frame, where a miniaturized fluorescence microscope (Inscopix) attached to a magnetic baseplate (Inscopix) were lowered to the top of the implanted GRIN lens, until a sharp image of anatomical landmarks (blood vessels) and putative neurons appeared in the focal plane. Baseplate was then cemented to the original head cap, allowing to fix the set focal plane for imaging.
