100 μg/ml ampicillin. After overnight incubation, all colonies were dislodged from the plates by adding 2 ml LB-broth containing 100 μg/ml ampicillin and agitation using 10–20 ColiRollersTM glass beads (EMD Millipore, Billerica, MA, United States). The colonies harvested from the six plates in LB-broth were pooled together. The liquid culture was incubated at 37°C for 5 h after which plasmids were isolated using 10 QIAprep® Spin miniprep columns (Qiagen, Germantown, MD, United States) as per manufacturer’s instructions. Column elutions were combined to create the plasmid library that was used for downstream experiments. The plasmid DNA concentration was determined using a Quant-iTTM DNA Broad Range kit (Thermo Fisher Scientific, Waltham, MA, United States).
