Chunk #93 — Results and discussion — Analysis of Akt/mTOR phosphoprotein pathway throughout the brain after chronic alcohol: Decreased expression of multiple mTOR/Akt phosphoproteins in 3xTg-AD alcohol-exposed mice (1-month post alcohol) — Hippocampus
In the 3xTgAD mice, prior studies have found decreased levels of IRS-1 in the membrane fraction of hippocampal lysates (Ma et al., 2009). Moreover, phosphorylated IRS-1 is increased in 9-month-old 3xTg-AD mouse HPC and cortex but decreased at 10 and 15 months of age (Velazquez et al., 2017) when neural and behavioral pathology is most severe. Thus, the reduction in hippocampal IRS-1 phosphorylation seen in 8-month-old 3xTg-AD mice in the present study suggests that alcohol drinking may have exacerbated the onset or magnitude of AD-like pathology. This effect of alcohol was measured 1month after exposure but is consistent with reduced IRS-1 phosphorylation seen after ethanol (100mM) in cell culture (Xu, Bhavani, Wands, & de la Monte, 1995).
