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Chunk #156 — STAR★Methods — Quantification and Statistical Analysis — Spatial correlation analysis

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Molecular Architecture of the Mouse Nervous System.
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Our aim here is the try and map the gene expression profile at the cluster level to the mouse in situ hybridization atlas of the Allen Institute for Brain Research (http://mouse.brain-map.org) (Lein et al., 2007). The Allen Mouse Brain Atlas was summarized into a 200 μm voxel dataset, providing the gene expression profile (all genes) for each voxel. In this analysis we used simple correlation between the voxel gene expression (from in situ hybridization) and the cluster gene expression profile (from scRNaseq).