Summary statistics from the primary MDD-META meta-analysis were loaded into Functional Mapping and Annotation of Genome-Wide Association Studies (FUMA GWAS) to test for gene-level associations using Multi-Marker Analysis of GenoMic Annotation (MAGMA).47 Input SNPs were mapped to 17,927 protein coding genes. The GWS threshold for the gene-based test was therefore determined to be p = 0.05/17,927 = 2.79×10-6. Genes from MAGMA’s gene-based association were used for gene ontology and drug-set enrichment using the ShinyGO18 web tool.
