By Affymetrix Clarion D Human microarray analysis of the 96 selected Set 2 samples (from 6 brain regions), we identified six differentially expressed (absolute FC > 2.0 & P < 0.001) coding genes (or mRNAs) in one or more brain regions (upregulated OCLN and downregulated GLS2 in AMY; downregulated SLC47A1, PROX1, MYO5B, and TNC in HIP; and upregulated OCLN in PUT) of AUD subjects (Fig. S9 and Table S6). Decreased expression of TNC was also observed in multiple brain regions of Set 1 AUD subjects (AMY: 2.53-fold decrease & P = 0.016; CN: 1.48-fold decrease & P = 0.307; HIP: 1.38-fold decrease & P = 0.406; and PFC: 2.61-fold decrease & P = 0.016).
