We also examined ethanol-induced mRNA transcriptome changes in hESC-derived cortical interneurons by mRNA-seq. A 7-day ethanol exposure did not cause coding gene expression changes at the above significance level (absolute FC > 2.0 & P < 0.001) (Fig. S10). When the significance level was set at FC > 2.0 & P < 0.01, 19 coding genes showed differential expression, and all of them were downregulated after a 7-day ethanol exposure (Table S7).
