Venn diagrams were used to show the number of differentially expressed mRNAs (P < 0.05) shared between eight brain regions of AUD subjects (Set 1 and Set 2) and ethanol-exposed hESC-derived cortical interneurons (Fig. S11). The in vitro cellular model study confirmed a number of AUD-associated mRNAs, including 15 mRNAs in the AMY, 15 mRNAs in the CN, five mRNAs in the CRB, five mRNAs in the HIP, seven mRNAs in the NAc, eight mRNAs in the PFC, 10 mRNAs in the PUT, and four mRNAs in the VTA. Among them, 13 AUD-associated coding genes identified in multiple brain regions were found differentially expressed in ethanol-exposed hESC-derived cortical interneurons (Fig. S11).
