The function of the top 19 differentially expressed (absolute FC > 2.0 & P < 0.05) miRNAs (Table S2) identified in one or more of the eight brain regions of AUD subjects (Set 1) was annotated by DIANA-mirPath. The top 14 KEGG pathways (P < 0.0001), including Morphine Addiction (P = 3.1 × 10−8; 15 miRNAs), Cocaine Addiction (P = 1.5 × 10−5; 15 miRNAs), and Amphetamine Addiction (P = 1.0 × 10−4; 15 miRNAs), were associated with mRNAs potentially targeted by these 19 miRNAs (Fig. 3). The function of the top 97 differentially expressed (absolute FC > 2.0 & P < 0.001) mRNAs (Table S5) identified in one or more of the eight brain regions of AUD subjects (Set 1) was annotated by DAVID. The Gene Ontology (GO) enrichment analysis showed that several molecular functions (MF; such as Phosphatidate Phosphatase Activity), biological processes (BP; such as Central Nervous System Myelination), and cellular components (CC; such as Integral Component of Membrane) were enriched in these 97 differentially expressed mRNAs. GO terms (MF, BP, and CC) over-represented (P < 0.05) for this gene set are displayed in Fig. S12.
