Differentially expressed and negatively correlated miRNA–mRNA pairs were included in brain region-specific IPA network analysis. The differential expression analysis P value was set at <0.05 and the absolute FC was set at >1.3 for both miRNAs and mRNAs. AUD-associated miRNA–mRNA-pathway networks for each of the eight brain regions (the Set 1 sample) are shown in Figs. 4 and 5, respectively. Within the AMY, 13 miRNAs (6 upregulated and 7 downregulated) and 13 paired mRNAs (10 upregulated and 3 downregulated) could regulate four pathways (CREB Signaling in Neurons, STAT3 Pathway, IL-8 Signaling, and Axonal Guidance Signaling) (Fig. 4a). Within the CN, 10 miRNAs (6 upregulated and 4 downregulated) and eight paired mRNAs (3 upregulated and 5 downregulated) could regulate four pathways (CREB Signaling in Neurons, Gap Junction Signaling, Axonal Guidance Signaling, and Neuroinflammatory Signaling) (Fig. 4b). Within the CRB, seven miRNAs (2 upregulated and 5 downregulated) and nine paired mRNAs (8 upregulated and 1 downregulated) could regulate three pathways (Synaptogenesis Signaling, CREB Signaling in Neurons, and Neuroinflammatory Signaling) (Fig. 4c). Within the HIP, 21 miRNAs (13 upregulated and 8 downregulated) and
