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Chunk #15 — Materials and methods — Data analyses

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The α2-adrenergic receptor agonist, clonidine, reduces alcohol drinking in alcohol-preferring (P) rats.
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In Study 1, alcohol intakes during drug treatments were analyzed by 2-way mixed analysis of variance (ANOVA) with repeated measures (dose × day with repeated measures on day) followed, when justified by determination of significant main effects or interactions in the ANOVA, by pairwise multiple comparisons using Fisher’s least significant difference (LSD) tests. Alcohol intakes on 2 post-treatment days were likewise analyzed by 2-way mixed ANOVA with repeated measures on day, followed by pairwise multiple comparisons with Fisher’s LSD tests. Alcohol intakes within the pre-treatment day were similarly analyzed, but with 1-way ANOVA. As discussed in the Results section, Kruskal-Wallis 1-way ANOVA on ranks was used to analyze water intake. In Study 2, saccharin intakes on the 3 treatment days were likewise analyzed by 2-way mixed ANOVA with repeated measures on day, followed by pairwise multiple comparisons with Fisher’s LSD tests. All analyses were conducted using the SigmaStat 3.5 program (Systat Software, Inc., Chicago, IL) with significance accepted at p < 0.05. Data are presented as mean ± SE.